The reserve carries around 1,600 individual large mammals. Cheetah, blesbuck, black wildebeest, red hartebeest, eland, common zebra, waterbuck, reedbuck, springbuck, mountain reedbuck, steenbok, grey duiker, oribi, leopard, buffalo, and hippopotamus can all be found in the reserve. There are also 4 lionesses and 2 male lions kept in a separate enclosure on the reserve. In October 2019, the Rietvlei lions were killed by poachers and since then, no lions have been kept at the reserve.